Internal Medicine and Medical Investigation Journal

ISSN: 2474-7750

Antibacterial Activities of 5-Nitro-2-uryl and 5-Nitro-2-Imidazolyl Derivatives of 1,3,4-Thiadiazole


Author(s): Mohammad Hassan Moshafi , Ali Peymani , Alireza Foroumadi , Mohammad Reza Zabihi , Farzad Doostishoar

Nitrofurans and nitroimidazoles are broad-spectrum antimicrobial

agents, which affect the microbial DNA. The aim of the present study was to evaluate

the new derivatives of these two groups of antimicrobials against certain Gram-positive

and Gram-negative bacterial strains.

Materials and Methods:

Seven new derivatives

of nitrofurans and nitroimidazoles were synthesized, and 6.4 mg of each derivative was

dissolved in dimethyl sulfoxide. Then, 8 serial dilutions (0.5, 1, 2, 4, 8, 16, 32, and 64

μg/ml) of each derivative was prepared using Muller-Hinton broth, and the minimum

inhibitory concentration for each derivative was measured and compared to ciprofloxa-

cin (standard).


All the derivatives had no antibacterial effects against

Gram-negative bacteria (minimum inhibitory concentration > 64 μg/ml); only 2-(5-ni-

tro-2-furyl)-5-(n-pentylsulfunyl)-1,3,4-thiadiazole exhibited mild antibacterial effects

against Klebsiella pneumonia (minimum inhibitory concentration of 16-32 μg/ml). The

antibacterial effects of the derivatives against Gram-positive bacteria also showed

variations from complete inhibition of the growth of Staphylococcus epidermidis and

Bacillus subtilis (minimum inhibitory concentration < 0.5 μg/ml) by 2-(5-nitro-2-fu-

ryl)-5-(n-buthylthio)-1,3,4-thiadiazole to no inhibition of S. epidermidis and streptococ-

cus pyogenes.


These compounds have weak antibacterial effects; only

two derivatives showed antibacterial effects similar to that of the positive control

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